Composite

Part:BBa_K3385063:Design

Designed by: Daniel Bavnh�j   Group: iGEM20_DTU-Denmark   (2020-10-16)


T1G3R_SeglA_glaA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1858
    Illegal EcoRI site found at 5747
    Illegal EcoRI site found at 7175
    Illegal XbaI site found at 1623
    Illegal XbaI site found at 5778
    Illegal SpeI site found at 1097
    Illegal PstI site found at 893
    Illegal PstI site found at 4009
    Illegal PstI site found at 6585
    Illegal PstI site found at 8319
    Illegal PstI site found at 8920
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1858
    Illegal EcoRI site found at 5747
    Illegal EcoRI site found at 7175
    Illegal NheI site found at 4886
    Illegal SpeI site found at 1097
    Illegal PstI site found at 893
    Illegal PstI site found at 4009
    Illegal PstI site found at 6585
    Illegal PstI site found at 8319
    Illegal PstI site found at 8920
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1858
    Illegal EcoRI site found at 5747
    Illegal EcoRI site found at 7175
    Illegal BglII site found at 3864
    Illegal BglII site found at 5386
    Illegal BglII site found at 8417
    Illegal BamHI site found at 1729
    Illegal BamHI site found at 2157
    Illegal BamHI site found at 4844
    Illegal BamHI site found at 5646
    Illegal BamHI site found at 5772
    Illegal BamHI site found at 6323
    Illegal XhoI site found at 548
    Illegal XhoI site found at 735
    Illegal XhoI site found at 963
    Illegal XhoI site found at 6183
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1858
    Illegal EcoRI site found at 5747
    Illegal EcoRI site found at 7175
    Illegal XbaI site found at 1623
    Illegal XbaI site found at 5778
    Illegal SpeI site found at 1097
    Illegal PstI site found at 893
    Illegal PstI site found at 4009
    Illegal PstI site found at 6585
    Illegal PstI site found at 8319
    Illegal PstI site found at 8920
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1858
    Illegal EcoRI site found at 5747
    Illegal EcoRI site found at 7175
    Illegal XbaI site found at 1623
    Illegal XbaI site found at 5778
    Illegal SpeI site found at 1097
    Illegal PstI site found at 893
    Illegal PstI site found at 4009
    Illegal PstI site found at 6585
    Illegal PstI site found at 8319
    Illegal PstI site found at 8920
    Illegal NgoMIV site found at 2566
    Illegal NgoMIV site found at 5104
    Illegal NgoMIV site found at 8307
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3392
    Illegal BsaI site found at 3530
    Illegal BsaI site found at 4219
    Illegal BsaI.rc site found at 4712
    Illegal SapI site found at 8808
    Illegal SapI site found at 9117


Design Notes

Full construct made using non-overlapping primers, which can pose difficulties on PCR amplification and subsequently USER assembly.

Source

Sequences were obtained using aspgd.org[1] and SingalP-5.0[2].

References

[1] Cerqueira GC, Arnaud MB, Inglis DO, Skrzypek MS, Binkley G, Simison M, Miyasato SR, Binkley J, Orvis J, Shah P, Wymore F, Sherlock G, Wortman JR (2014). The Aspergillus Genome Database: multispecies curation and incorporation of RNA-Seq data to improve structural gene annotations. Nucleic Acids Res 42 (1); D705-10.

[2] SignalP 5.0 improves signal peptide predictions using deep neural networks. José Juan Almagro Armenteros, Konstantinos D. Tsirigos, Casper Kaae Sønderby, Thomas Nordahl Petersen, Ole Winther, Søren Brunak, Gunnar von Heijne and Henrik Nielsen. Nature Biotechnology, 37, 420-423, doi:10.1038/s41587-019-0036-z (2019)